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CO2 Incubator Sterilization method with 90ºC Moist Heat Decontamination 17 Jun 2010: In this application note, Esco validate the Swiftcon 90ºC moist heat decontamination cycle of the Celculture CO2 Incubator by performing decontamination tests on 5 bacterial suspension preparations, including Aspergillus niger and Bacillus subtilis. After decontamination, the microbial suspension discs were placed on tubes containing PBS which were assayed by culturing methods to quantitate Total Viable Count at each location. Esco Micro Pte Ltd
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Stem Cell-Derived Human Neurons in CNS Drug Discovery 07 Jun 2010: In this application note from Molecular Devices reproducible batches of human neurons are generated that will significantly improve and impact CNS drug discovery. The stem cell-derived neurons express all known markers typically associated with mature neurons and are functionally active. These neurons have been used in a recent screen to identify antagonists of a novel GPCR target implicated in schizophrenia. Molecular Devices
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Cytotoxicity in Cells: Easy Determination Using Lonza ViaLight Plus and ToxiLight BioAssays on the SpectraMax L Microplate Luminometer 07 Jun 2010: The Lonza ViaLight® Plus and ToxiLight® Bioluminescent Cytotoxicity Assays exploit the cell characteristics of ATP levels and adenylate kinase release respectively, as cytotoxicity measures and enable detection using bioluminescent methods. This application note demonstrates that the SpectraMax L Microplate Luminometer and SoftMax Pro Software from Molecular Devices combine well with both assays providing a fast and sensitive combination of technologies in the field of cell death. Molecular Devices
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Are Natural Products Delivering? Experiences and Recommendations from 27 Screening Campaigns 04 May 2010: In this application note from BioFocus, microbial strain subfractions are generated and used in high-throughput screens (HTS) for analysis with regard to diversity, selectivity and strain origin. Natural compounds were identified during the follow up of subfraction screening programs and their chemical properties have been analyzed. Searches in natural product databases revealed that 135 of the 645 identified natural compounds are novel compounds. BioFocus
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Matrix Dedicated Evidence Biochip Array Based Immunoassays for Toxicological and Forensic Applications 05 Apr 2010: This application note demonstrates examples of the applicability of biochip arrays for the simultaneous detection of multiple drugs of abuse per sample of urine, blood or oral fluid using matrix dedicated kits. This technology has also been applied to tissue and meconium. Randox Laboratories Ltd
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Direct Count of White Blood Cells from Peripheral Blood Sample without Lysing Red Blood Cells 09 Jul 2009: The concentration of white blood cells in peripheral blood is routinely used in the research of various diseases including bacteria/virus infections, leukaemia and leucopenia. Here an alternative is proposed to the traditional time consuming methods of quantitative white blood cell analysis. The DNA dye acridine orange is used to specifically stain white blood cells of a diluted whole blood sample. Images are gained and analysed using the Cellometer Vision. Nexcelom Bioscience
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Empore Extraction Disks used to purify and concentrate a novel immunomodulator drug 07 Jan 2009: This application note demonstrates the use of large diameter (47 mm) Empore Extraction Disks to purify and concentrate a novel immunomodulator drug, Imiquimod (AldaraTM), and its metabolites from urine. 3M Bioanalytical
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In vitro diagnostics (IVD) compliance and what it means in practice
17 Jun 2008: In Vitro Diagnostics (IVD) is the analysis of medical samples, such as blood, tissues or urine, taken from patients or healthy individuals. All equipment used for IVD, including pipettors and tips, must be certified with the CE mark according to the In Vitro Diagnostic (IVD) Directive (98/79/EC). Biohit
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Protecting your sample - using a certified pipette tip
10 Jun 2008: Clean, high quality pipettor tips are critical in life science research. Typical contaminants, which can originate inside and outside the laboratory, are endotoxins and enzymes, namely RNase and DNase. This technical paper explains how the quality of the tip and the manufacturing process are critical to ensure that equipment is free from endotoxins and enzymes which are difficult or impossible to remove. Biohit
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